Audio is played using the transport controls, which appear in the third column of a Mixere document, under the heading "Transport". The controls consist of four buttons:
| Play | Plays the audio from its current position. |
| Pause | Stops the audio without rewinding it. |
| Stop | Stops the audio and rewinds it. |
| Loop | Turns loop mode on/off. Normally, a track plays until it reaches the end of the audio file, and then stops by itself. However, in loop mode, the audio plays continuously. |
Various shortcuts are available: